diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. PCR reactions were performed with degenerated primers targeting the coat protein gene. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. Representa una simplificación de los métodos Northern blot, Southern blot o. PVY, PVS and PLRV viruses. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. Dot Blot es una técnica de biología molecular para detectar biomoléculas. duo), el protocolo completo consume de 3 a. Para ello se nucleic acid hybridization. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). PCR, Northern blot y Dot blot. 'protocolo proporcionado por la Dra. I RNA. . luminescent detection for hybridization. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. The hybridization. General detection was achieved by DNA hybridization with a PHV-A probe. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and.